Page 53 - A Magyar Szemorvostársaság 2022. évi kongresszusának programja és az előadások kivonata

Elődáskivonatok/Abstracts

Effects of different smoke extracts on retrobulbar fibroblasts
measured by real time cell electronic sensing technique
János Aranyosi , Erika Galgóczi , Mónika Katkó , Annamária Erdei , Mariann Fodor , Zoltán Ujhelyi ,
1
2
2
1
3
2
Endre Nagy V , Bernadett Ujhelyi 1
2
1 University of Debrecen, Faculty of Medicine, Department of Ophthalmology, Debrecen
2 University of Debrecen, Faculty of Medicine, Department of Endocrinology, Debrecen
3 University of Debrecen, Faculty of Pharmacology, Department of Techology, Debrecen
Introduction: Cigarette smoke exposure is a known risk factor for thyroid associated orbitopathy (TAO), with serious effect on disease se-
verity and response to treatment. Nowadays, alternative forms of smoking, such as heated tobacco products (HTPs) or e-cigarettes (ECIGs),
are becoming more common alongside traditional cigarettes (CCs).
Aim: Analysis of primary fibroblast cell culture from tissue removed from TAO and healthy orbits with the addition of smoke extracts from
different tobacco products.
Methods: Primary cell cultures were obtained from tissue collected during orbital surgery. Cells were examined for 72 hours with continuous
measurement in DMEM medium by adding tobacco smoke extracts (SE), which were prepared according to previous publications. The
metabolic activity of the cells was assessed by MTT assay and hyaluronan (HA) production by ELISA at 24, 48, 60 and 72 h after the
addition of extracts.
Results: Untreated TAO cells had the lowest cell index (CI) value (CI max =3.3). CC treated TAO CI values peaked at 5.38. HTP and
ECIG treated TAO cells behaved similarly and reached higher values compared to CC treated cells. ECIG treated TAO cells had CI max =6.33
and HTP treated TAO cells had CI max =6.25. TAO cells treated with CC, HTP and ECIG all showed significant differences (p<0.0001).
The CI values show a time-dependent, dynamic change in cellular activity, with ECIG causing the largest CI change in TAO fibroblasts.
There was no significant difference in metabolic activity in any of the SE treated cell lines compared to untreated cells. The basal metabolic
activity of untreated GO and NON-GO cells did not differ. No difference was found in HA production between treated and untreated cells.
Conclusion: The orbital fibroblasts responded differently to CC, HTP and ECIG smoke extracts and cells from TAO samples were more
sensitive to tobacco smoke treatments than normal fibroblasts. The current model may be useful for designing further in vitro researches to
investigate the pathogenesis of TAO, and the negative effects of smoking.

Száraz szem betegség: újdonságok a kutató laboratóriumtól
a klinikumig
Tóth-Molnár Edit
Szegedi Tudományegyetem Szemészeti Klinika, Szeged
A száraz szem betegség mára a fejlett országok meghatározó szemészeti problémájává vált. A betegség okozta társadalmi-gaz-
dasági kihívások miatt a területen folyó intenzív kutatás-fejlesztés számos fontos előrelépést jelentő újdonságot eredménye-
zett mind a diagnosztika, mind a terápia területén. A kurzus előadója áttekinti a diagnosztikus szemlélet változásait, a vál-
tozások technikai követéseként fejlesztett új eszközök nyújtotta lehetőségeket, továbbá bepillantást nyújt saját kutatásaik
jövőbeni gyógyszerfejlesztési és klinikai felhasználásra is alkalmasnak ítélt eredményeiről.
Tóth-Molnár Edit: Szemléletváltás a száraz szem diagnosztikájában: a preocularis könnyfilm és
Meibom-mirigyek komplex, noninvazív vizsgálata
Tóth-Molnár Edit: Száraz szem betegség a transzlációs medicina tükrében: új eredmények és lehetőségek

Dry eye disease: novelties from the bench to bedside
Edit Tóth-Molnár
University of Szeged, Department of Ophthalmology, Szeged

Dry eye disease has become an emerging problem of ophthalmology in the industrialized countries. Socio-economic difficulties caused by
the disease positioned this morbidity in the front line of ophthalmic research and development resulting in numerous new outcomes both in
the diagnostic and therapeutic fields. The course will review the latest innovations of diagnosis and the new approaches of dry eye disease
enabled by the new diagnostic devices. The author will also give an overview of her own latest scientific results which might be in the scope
of pharmacological or clinical utilization in the future.

Edit Tóth-Molnár: change of approch in the diagnostic of dry eye disease: complex non-invasive examination of
preocular tear film and Meibom gland function
Edit Tóth-Molnár: Dry eye disease in the light of translational medicine: new results and potentials

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