Page 63 - Magyar Gasztroenterológiai Társaság 2020. november 6–7. – ONLINE KONGRESSZUS

Előadáskivonatok / Abstracts

7. Division of Haematology, First Department of Medicine, Methods: The Hungarian Pancreatic Study group has
University of Pécs Medical School prospectively collected multicenter clinical data of 1435 adult
patients between 2012 and 2017. 1429 of them contained
Introduction: The goal of treatment in ulcerative colitis (UC) valuable data on pancreatic necrosis and were enrolled.
is to induce and maintain remission. The addition of Statistical analyses compared pancreatitis with (ANP) and
granulocyte and monocyte apheresis (GMA) to conventional without necrosis (AP). Predictive models were built by the
therapy may be a promising therapeutic alternative. Random Forest approach.
Aims: In this meta-analysis, we aimed to assess the efficacy Results: 9.31% (n=133) of the patients had ANP. As
and safety profile of GMA. expected ANP was associated with higher mortality (8.27%
Methods: We searched four databases for randomized or vs 1.93%; p<0.0001), more severe disease (mild: 0.00% vs
minimized controlled trials which discussed the impact of 75.69%, moderate: 73.68% vs 20.91%, severe: 26.32% vs
additional GMA therapy on clinical remission induction and 3.40%), longer hospitalization (22.95±19.23 days vs
clinical remission maintenance compared to conventional 10.18±7.22 days; p<0.0001), and higher rate of
therapy alone. Odds ratios (OR) with 95% confidence complications (pseudocyst: 30.83% vs 6.34%, p<0.0001;
intervals were calculated. The random-effects model was diabetes: 13.53% vs 3.24%, p<0.0001; respiratory failure:
used to pool effect sizes. Heterogeneity was tested by 20.45% vs 3.27%, p<0.0001); heart failure: 8.33% vs 1.17%,
calculating Higgins’ I indicator. p<0.0001); renal failure: 15.19% vs 1.71% , p<0.0001).
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Results: A total of eleven studies were eligible for meta- Several risk factors were identified among on admission
analysis. GMA was clearly demonstrated to induce and parameters. After combining these parameters, we created a
maintain clinical remission more effectively than conventional predictive model with the Random Forest approach that does
therapy alone (598 patients: OR: 1.93, CI: 1.28–2.91, not include false negative cases.
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p=0.002, I =0.0% for induction; 71 patients: OR: 8.34, CI: Conclusion: Pancreatic necrosis markedly influences the
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2.64–26.32, p<0.001, I =0.0% for maintenance). Although outcome of acute pancreatitis. Without the presence of false
reporting was diverse across studies, the frequency of negative cases, our model is able to rule out development of
adverse events did not differ between groups. ANP in this derivation cohort. After the validation of our result,
Conclusion: GMA appears to be more effective as an we will translate it into a predictive bioinformatics tool to help
adjunctive treatment in inducing and maintaining remission physicians in assessing risk of this severe complication.
in UC patients than conventional therapy.
79. THE ORGANOSULFUR DIMETHYL TRISULFIDE MAY
78. RISK PREDICTION MODEL FOR DEVELOPING ACT AS AN ANTIOXIDANT TO REDUCE THE SEVERITY
PANCREATIC NECROSIS OF EXPERIMENTAL ACUTE PANCREATITIS
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Kiss S. 1,2,3 , Farkas N. , Fehérvári P. , Pecze L. , Földi M. 1,2,3 , Kormányos E. , Balla Z. , Fűr G. , Bálint E. , Totonji A. ,
Vincze Á. , Gódi S. , Bajor J. , Czimmer J. , Sarlós P. , Bátai Z. , Pozsgai G. , Börzsönyi Á. , Hegyi P. , Pintér E. ,
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Hágendorn R. , Takács T. , Izbéki F. , Halász A. , Hamvas Rakonczay Jr Z. , Kiss L.
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J. , Varga M. , Crai S. , Mickevicius A. , Patai Á. , Ihász 1. Department of Pathophysiology, University of Szeged,
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M. , Varjú P. , Faluhelyi N. , Farkas O. , Miseta A. , Szeged, Hungary; 2. Department of Pharmacology and
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Kelemen D. , Papp R. , Hegyi P. , Szentesi A. , Párniczky Pharmacotherapy, University of Pécs, Pécs, Hungary; 3.
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A. , Hegyi P. 1,3,5 Institute for Translational Medicine, University of Pécs, Pécs,
1. First Department of Internal Medicine, University of Hungary
Szeged, Szeged, Hungary; 2. Doctoral School of Clinical
Medicine, University of Szeged, Szeged, Hungary; 3. Introduction: Acute pancreatitis (AP) is an inflammatory
Institute for Translational Medicine, University of Pécs disease which doesn’t have any adequate therapy. Our
Medical School, Pécs, Hungary; 4. Division of previous studies have shown that administration of dimethyl
Gastroenterology, First Department of Medicine, University trisulfide (DMTS), a member of the organic trisulfide family,
of Pécs Medical School, Pécs, Hungary; 5. Division of reduces the severity of AP.
Translational Medicine, First Department of Medicine, Aims: to investigate the effects of DMTS on AP and to reveal
University of Pécs Medical School, Pécs, Hungary; 6. First its mechanism of action.
Department of Medicine, University of Pécs Medical School, Methods: AP was induced in FVB/n mice by hourly
Pécs, Hungary; 7. Szent György University Teaching intraperitoneal injections of 10x50µg/kg cerulein. At the same
Hospital of Fejér County, Székesfehérvár, Hungary; 8. time DMTS was administered subcutaneously 3-hourly in
Péterfy Hospital, Budapest, Hungary; 9. Dr. Réthy Pál different doses. AP severity was evaluated by histological
Hospital, Békéscsaba, Hungary; 10. Pándy Kálmán Hospital scoring. Primary mouse pancreatic acinar cells were isolated
of Békés County, Gyula, Hungary; 11. Vilnius University by collagenase digestion and were used for in vitro assays
Hospital Santaros Clinics, Vilnius, Lithuania; 12. where DMTS was applied between 0.1-100µg/ml
Markusovszky University Teaching Hospital, Szombathely, concentrations. MTT and propidium iodide were utilized to
Hungary; 13. Department of Radiology, University of Pécs determine acinar viability. Reactive oxygen species (ROS)
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Medical School, Pécs, Hungary; 14. Department of and intracellular Ca concentration ([Ca ]i) measurements
Laboratory Medicine, University of Pécs Medical School, were determined by microfluorimetry.
Pécs, Hungary; 15. Department of Surgery, University of Results: 2x75 and 2x100mg/kg DMTS significantly
Pécs Medical School, Pécs, Hungary; 16. Heim Pál National ameliorated cerulein-induced AP severity. 3-60 µg/ml DMTS
Institute of Pediatrics, Budapest, Hungary concentrations increased the acinar metabolic activity and
<100 µg/ml DMTS did not affect cell viability or necrosis
Introduction: Necrosis is a major local complication in acute within 1 hour. DMTS alone did not induce ROS production,
pancreatitis which affects its outcome. but it markedly reduced the ROS signal in cases of
Aims: Our aim was to evaluate the clinical characteristics of menadione and H2O2 administration. DMTS treatment did not
acute necrotizing pancreatitis (ANP) and to design a influence the [Ca ]i compared to the control.
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predictive model for that. Conclusion: DMTS administration significantly alleviated
the severity of experimental AP. DMTS itself did not affect
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Central European Journal of Gastroenterology and Hepatology 61
Volume 6, Supplementum 2 / November 2020