Page 64 - Magyar Gasztroenterológiai Társaság 2020. november 6–7. – ONLINE KONGRESSZUS

Előadáskivonatok / Abstracts

acinar viability, [Ca ]i and ROS production. However, it the National Research, Development and Innovation Office,
2+
showed strong protection against oxidative stress. Our by the Ministry of Human Capacities (EFOP 3.6.2-16-2017-
results suggest that DMTS has anti-inflammatory effects and 00006).
further investigation is warranted in AP.
Acknowledgements: NKFIH-PD-129114, NKFIH-K-119938, 81. THE USE OF EARLY ACHIEVABLE SEVERITY (EASY)
GINOP-2.3.2-15-2016-00034, GINOP-2.3.2-15-2016-00048, INDEX IS BENEFICIAL FOR RAPID RISK
and EFOP-3.6.2-16-2017-00006. STRATIFICATION IN ACUTE PANCREATITIS AT
HOSPITAL ADMISSION
80. ESOPHAGEAL ORGANOID CULTURE IS A NOVEL Kui B. , Gódi S. , Bajor J. , Török I. , Macaria M. , Farkas
4
4
1,2
2,3
2,3
MODEL TO STUDY EPITHELIAL ION TRANSPORT H. , Mickevicius A. , Sallinen V. , Maldonado E. , Papp M. ,
8
7
5
6
4
MECHANISMS Kovács G. , Fehér E. , Sarlós P. , Galeev S. , Németh B. ,
8
1
2,3
9
8
2
1
11
11
Korsós M. , Bellák T. , Becskeházi E. , Venglovecz V. Vincze Á. , Pencik P. , Ozola-Zalite I. , Pukitis A. , Litvin
2,3
1
1
10
1. Department of Pharmacology and Pharmacotherapy, A. , Kovalskaya I. , Czimmer J. , Pár G. , Hritz I. , Illés
2,3
13
2,3
12
14
University of Szeged; 2. Department of Anatomy, Histology D. , Szentesi A. , Lakatos L. , Capurso G. , Patai Á. ,
15
17
1
16
1,2
13
18
20
and Embryology, University of Szeged Stilidi E. , Peserico G. , Zadorozhna K. , Sonmez E. ,
19
22
20
23
21
Tüzün Ince A. , Vishalkumar S. , Horibe M. , Ihász M. ,
2
2
2
2
2,3
Introduction: Esophageal epithelial cells (EECs) protect the Erőss B. , Márta K. , Szakács Z. , Farkas N. , Gede N. ,
24
24
25
lower layers during esophageal reflux. One of the major Halász A. , Gajdán L. , Izbéki F. , Poropat G. , Robu G. ,
24
26
components of the epithelial defensive mechanisms is the Jinga M. , Hegyi P. 1,2,3
26
ion transport processes, however their role under 1. University of Szeged, First Department of Medicine,
pyhsiological and pathophysiological conditions is not Szeged, Hungary; 2. University of Pécs, Medical School,
completely clear. One of the reason for this is the lack of good Department of Translational Medicine, Pécs, Hungary; 3.
experimental models on which the functional changes of University of Pécs, Medical School, First Department of
EECs can be investigated. Medicine, Pécs, Hungary; 4. Mures County Emergency
Aims: Therefore, our aim in this study was to generate Hospital, Department of Gastroenterology, Targu Mures,
esophageal organoid cultures (EOCs) from epithelial tissue Romania; 5. Vilnius University Hospital Santariskiu Klinikos,
of mice and to investigate the presence of ion transporters Department of Gastroenterology, Hepatology and Nutrition,
on them. Vilnius, Lithuania; 6. Hospital of Helsinki University Central
Methods: EOCs were isolated from 8-20 weeks old mice, Hospital, Department of Abdominal Surgery, Helsinki,
using three different mice strains (CD1, C57/Bl6 and FVB/N). Finland; 7. Consorci Sanitori del Garraf, Sant Pere de Ribes,
Changes in intracellular pH (pHi) was measured using Department of General Surgery, Sant Pere de Ribes, Spain;
microfluorometry and the pH-sensitive fluorescence dye, 8. University of Debrecen, Department of Internal Medicine,
BCECF-AM. For determining the resting pHi, the high Debrecen, Hungary; 9. SaintLuke Clinical Hospital, Saint-
K /nigericin technique was used whereas buffering capacity Petersburg, Department of Digestive Surgery,
+
was measured by the ammonium prepulse technique. For SaintPetersburg, Russian Federation; 10. Vitkovicka
immunostaining, EOCs were fixed in 4% PFA and Nemocnice A. S., Centrum Pece O Zazivaci Trakt, Ostrava,
dehydrated in 30% saccharose for 3 days before embedding Czech Republic; 11. Pauls Stradins Clinical University
in Cryomatrix and the presence of stem cells and the Hospital, Riga, Department of Gastroenterology, Riga,
TM
differentiated epithelial cells in the organoids was proved by Latvia; 12. Gomel Regional Clinical Hospital, Department of
Lgr5 and cytokeratin 14 (CK14) immunofluorescent staining. Surgery, Gomel, Belarus; 13. Bohomolets National Medical
Results: For the generation of EOCs esophagus was University, General Surgery, Kiev, Ukraine; 14. 1st
removed and digested with dispase II (1 mg/ml) for 40 min. Department of Surgery, Semmelweiss University, Budapest,
The mucosa was peeled from the submucosa, then Hungary; 15. County Hospital Veszprém, Department of
incubated with 0,25% trypsin in order to obtain individual Gastroenterology, Veszprém, Hungary; 16. S. Andrea
cells. Cells were then suspended in Matrigel® matrix and Hospital University "Sapienza", Digestive and Liver Disease
cultured for 10-14 days. In each mice, organoids had a three- Unit, Rome, Italy; 17. 2nd Department of Internal Medicine,
dimensional, approx. spheroidal structure, growing in the Semmelweiss University, Budapest, Hungary; 18. Hospital of
extracellular matrix. The average size Of EOCs were Medical Academy, Department of Gastroenterology,
between 50-150- micrometer. Their maximum size was Simferopol, Russian Federation; 19. University of Padua,
about 200 µm at the end of the second week, although Department of Surgery, Oncology and Gastroenterology,
differences in morphology and size have been observed. The Padua, Italy; 20. Hospital of Bezmialem Vakif, University
resting pHi, of CD1 mice was 7.47 ± 0.027. Microfluorometric School of Medicine, Department of Internal Medicine,
measurement showed the presence of functionally active Gastroenterology Clinic, Istanbul, Turkey; 21. Tan Tock Serg
+
Na /H exchanger (NHE) and Cl /HCO3 (CBE) transporters Hospital, Department of Surgery, Singapore, Singapore; 22.
+
-
-
on the EOCs. We have detected Lgr5 expressions in all cells Keio University, School of Medicine, Division of
of the organoids; whereas CK14 staining showed granular Gastroenterology and Hepatology Department of Internal
arrangement. Medicine, Tokyo, Japan; 23. Markusovszky University
Conclusion: We have successfully set up the culturing of Teaching Hospital, Department of Gastroenterology,
mice esophageal organoids and our preliminary results Szombathely, Hungary; 24. Fejér County Szent György
showed that EOCs express both alkalizing and acidifying University Teaching Hospital, Department of
transporters. We strongly belive that EOCs are a suitable, in Gastroenterology, Székesfehérvár, Hungary; 25. Clinical
vitro experimental model to study esophageal epithelial Hospital Center Rijeka, Department of Gastroenterology,
function and can also be used to investigate the Rijeka, Croatia; 26. Dr Carol DaVila Central Military
pathomechanism of reflux-induced esophageal diseases. Emergency Hospital, Department of Gastroenterology,
This study was supported by the National Research, Bucharest, Romania
Development and Innovation Office (FK123982) and the
Economic Development and Innovation Operative Introduction: Acute pancreatitis (AP) is one of the most
Programme Grants (GINOP-2.3.2-15-2016-00015) and by common diseases of the gastrointestinal tract associated
62

62 Central European Journal of Gastroenterology and Hepatology
Volume 6, Supplementum 2 / November 2020