Page 52 - Magyar Gasztroenterológiai Társaság 2020. november 6–7. – ONLINE KONGRESSZUS

Előadáskivonatok / Abstracts

Sensitivity Specificity PPV NPV 1. First Department of Medicine, University of Szeged; 2.
PRO RBS 97.5% 43.3% 72.2% 92% Department of Pharmacology and Pharmacotherapy,
0 vs MES 0 University of Szeged; 3. MTA-SZTE Translational
PRO RBS 96.1% 67.7% 90.7% 84% Gastroenterology Research Group, University of Szeged; 4.
0 vs MES Institute for Translational Medicine, University of Pécs; 5.
≤1 Momentum Translational Gastroenterology Research Group,
PRO RBS 97.3% 39% 66.7% 92% Hungarian Academy of Sciences, University of Szeged
0 vs Baron
0 Introduction: Acute pancreatitis(AP) is a severe disorder
PRO RBS 92.7% 70.8% 93.5% 68% with no specific treatment. Recent years, the cystic fibrosis
transmembrane conductance regulator(CFTR) has been
0 vs Baron shown to be a possible drug target in AP. We hypothesized
≤1 that restoration of CFTR expression and/or function can
PRO SF 0 93.8% 50.9% 74.3% 84.4% decrease the severity of AP.
vs MES 0 Aims: We aimed to investigate the effects of Ivacaftor(VX-
PRO SF 0 91.2% 74.2% 92.1% 71.9% 770) and Lumacaftor(VX-809) in guinea pigs.
vs MES ≤1 Methods: Pancreatic ducts(PDs) were isolated from guinea
PRO SF 0 93.2% 45.8% 68.3% 84.4% pigs. CFTR damage was induced by different concentration
vs Baron 0 of EtOH(30, 50 and 100mM) for 12hours. In order to
PRO SF 0 87.2% 75% 94.1% 56.2% understand the dose and time dependency, both drugs were
vs Baron administered in different, ascending concentrations(1, 3, 5,
≤1 10µM) for different period of times(3, 7, 9 hours). The
PRO2 96.2% 47.2% 73.3% 89.3% expression of CFTR was visualized by
remission immunohistochemistry and confocal microscopy. To study
vs MES 0 the effects of VX-770 and VX-809 inflammation was induced
PRO2 95.1% 74.2% 92.4% 82.1% by 10-times hourly intraperitoneal injections of 50µg/kg
remission cerulein. After the third injection animals were treated by per
vs MES ≤1 os 7,143mg/kg VX-770 and 8,929mg/kg VX-809.
PRO2 95.9% 42.4% 67.6% 89.3% Results: Administration of EtOH dose dependently
remission decreased the plasma membrane expression of CFTR. VX-
vs Baron 0 770 dose dependently restored the membrane localization of
PRO2 90.8% 75% 94.3% 64.3% CFTR damaged by 12h treatment of 30mM EtOH. The same
remission beneficial effect was seen by VX-809. Combination of the two
vs Baron drugs did not synergize each other effects significantly. 3h-
≤1 administration of both drugs already provided the maximum
partial 98.8% 43.4% 72.5% 95.8% effect. In vivo experiments revealed that oral administration
MAYO vs of VX-770 and VX-809 have a beneficial effect in AP by
MES 0 reducing oedema, necrosis, leukocyte infiltration and serum
partial 99% 74.2% 92.7% 95.8% amylase activity.
Conclusion: Correction of CFTR expression in AP
MAYO vs decreases disease severity.
MES ≤1
partial 98.6% 39% 67% 95.8% 47. INVESTIGATION OF PANCREATIC DUCTAL CELLS
MAYO vs USING PANCREAS SLICES
Baron 0 Gál E. , Dolensek J. , Stozer A. , Pohorec V. , Ébert A. ,
1
1
2
2
2,3
partial 94.5% 75% 94.5% 75% Venglovecz V.
1
MAYO 1. University of Szeged, Department of Pharmacology and
Baron ≤1 Pharmacoterapy; 2. Faculty of Medicine, University of
SCCAI vs 96.2% 45.3% 72.6% 88.9% Maribor, Maribor, Slovenia; 3. Faculty of Natural Sciences
MES 0 and Mathematics, University of Maribor, Maribor, Slovenia
SCCAI vs 96.1% 74.2% 92.5% 85.2%
MES ≤1 Introduction:: The tissue slice technique offers several
SCCAI vs 95.9% 40.7% 67% 88.9% benefits compared to isolated cells and cell clusters that
Baron 0 help us understand the (patho)physiology of several
SCCAI vs 91.7% 75% 94.3% 66.7% organs in situ. The most prominent features are preserved
Baron ≤1 architecture and function, with intact homotypic and
Conclusion: We found no difference across accuracy of heterotypic interactions between cells in slices. In the
RBS, SF, PRO2, partial Mayo and SCCAI in predicting pancreas, this technique has been utilized successfully to
endoscopic healing. A strong association was found with study acinar and endocrine islet cells. However, it has
high PPV for MES/Baron ≤1 and high NPV for MES/Baron 0. never been used to investigate ductal function.
FCAL, but CRP was not associated to clinical and Aims: Our aim was to use this technique to study PDEC
endoscopic remission. structure and function in situ.
Methods: C57BL/6 mice were used for preparation of
46. CORRECTION AND/OR ACTIVATION OF CFTR pancreas tissue slices. Low melting point agarose was
DECREASE THE SEVERITY OF ACUTE PANCREATITIS injected into the common bile duct and the whole organ was
5
5
1,2
Grassalkovich A. , Tóth E. , Maléth J. , Madácsy T. , extracted. For morphological studies, the tissues were
1
2
Venglovecz V. , Hegyi P. embedded in agarose and cryosectioned to obtain 15 µm
3,4
thick slices. To visualize pancreatic ducts, (i) the Giemsa
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50 Central European Journal of Gastroenterology and Hepatology
Volume 6, Supplementum 2 / November 2020