Page 73 - Magyar Gasztroenterológiai Társaság 2020. november 6–7. – ONLINE KONGRESSZUS

Előadáskivonatok / Abstracts

lemostuk. A lokális hatás rögtön látható volt, de a vérzések consistently supporting the antithrombotic effect of the
consistently supporting the antithrombotic effect of the
száma inkább szaporodott. gluten-free diet. The multivariate model confirmed these
lemostuk. A lokális hatás rögtön látható volt, de a vérzések
gluten-free
száma inkább szaporodott. associations. diet. The multivariate model confirmed these
Results: Ezt követően 2 ml, majd 10 ml 8%-os formaldehidet
Results: Ezt követően 2 ml, majd 10 ml 8%-os formaldehidet Conclusion: Impaired erythrocyte deformability may
alkalmaztunk 4-5 perces behatási idővel, 4 egymás utáni
associations.
contribute to the elevated cardiovascular risk for CeD
napon keresztül, ami jelentős javulást eredményezett. Egy
alkalmaztunk 4-5 perces behatási idővel, 4 egymás utáni Conclusion: Impaired erythrocyte deformability may
napon keresztül, ami jelentős javulást eredményezett. Egy contribute to the elevated cardiovascular risk for CeD
patients, while the gluten-free diet-dependent difference in
hónap múlva a kontroll során és további egy hónap múlva
hónap múlva a kontroll során és további egy hónap múlva patients, while the gluten-free diet-dependent difference in
erythrocyte aggregability further strengthens the importance
egy alkalommal alkalmaztunk 8% formalinos instillálást.
erythrocyte aggregabi
egy alkalommal alkalmaztunk 8% formalinos instillálást. of dietary adherence. lity further strengthens the importance
Ennek hatására a beteg vérzése megszűnt és több mint fél
Ennek hatására a beteg vérzése megszűnt és több mint fél of dietary adherence.
éve mesalazin kúp mellett nem ismétlődött. Kontroll vizsgálat
102. EFFECT OF DNA FRAGMENTS AND NUCLEOTID
éve mesalazin kúp mellett nem ismétlődött. Kontroll vizsgálat 102. EFFECT OF DNA FRAGMENTS AND NUCLEOTID
SUPPLEMENTATION ON TUMOR DEVELOPMENT IN
során a rektum nyálkahártya endoszkópos képe jelentősen
javult. a rektum nyálkahártya endoszkópos képe jelentősen
során SUPPLEMENTATION ON TUMOR DEVELOPMENT IN
MICE MODEL
javult. MICE MODEL 1 1 2 1
Nagy Z. , Zsigrai S. , Barták B. , Kalmár A. , Szigeti K. ,
Conclusion: A gyógyszeres kezelésre nem javuló
1
2 1
Valcz G. , Igaz P. , Tulassay Z. , Molnár B. A. , Szigeti K. ,
1
esetekben a formaldehid endoszkópos lokális nem javuló
Conclusion: A gyógyszeres kezelésre Nagy Z. , Zsigrai S. , Barták B. , Kalmár 2 2 1
2 1
2
2
2
2
2
esetekben a formaldehid endoszkópos lokális Valcz G. , Igaz P. , Tulassay Z. , Molnár B.
1. 2nd Department of Internal Medicine, Semmelweis
instillációja hatékony és biztonságos megoldást jelenthet az
instillációja hatékony és biztonságos megoldást jelenthet az 1. 2nd Department of Internal Medicine, Semmelweis
University, Budapest, Hungary; 2. Molecular Medicine
irradiációs proctitis esetében. A kezelés során a fájdalom volt
irradiációs proctitis esetében. A kezelés során a fájdalom volt University, Budapest, Hungary; 2. Molecular Medicine
Research Group, Hungarian Academy of Sciences,
a legjelentősebb mellékhatás, amit lokálisan alkalmazott
a legjelentősebb mellékhatás, amit lokálisan alk
Lidocaninnal nem tudtunk érdemben enyhíteni. almazott Research Group,
Budapest, Hungary Hungarian Academy of Sciences,
Lidocaninnal nem tudtunk érdemben enyhíteni. Budapest, Hungary
101. HAEMORHEOLOGICAL ALTERATIONS IN COELIAC Introduction: The balance of dNTP pool is needed for
101. HAEMORHEOLOGICAL ALTERATIONS IN COELIAC Introduction: The balance of dNTP pool is needed for
DISEASE: A CASE-CONTROL STUDY
normal DNA synthesis. Nucleotide depletion can cause DNA
DISEASE: A CASE-CONTROL STUDY 4 damage thus it could lead to tumor development. The
1,2
2,3
1,2
Nagy M. , Szakács Z. , Csiszár B. , Farkas N. , Kenyeres
normal DNA synthesis. Nucleotide depletion can cause DNA
Nagy M. , Szakács Z. , Csiszár B. , Farkas N. , Kenyeres damage thus it could lead to tumor development. The
2,3
1,2
P. , Erős A. , Sarlós P. , Alizadeh H. , Szentesi A. ,
2,6
supplementation of substrates that are involved in DNA
1
4
1,2
1,2
2,3
2,5
2,3
2,5 2,6
8 2,5
P. , Erős A. , Sarlós P. , Alizadeh H. , Szentesi A. 1 supplementation of substrates that are involved in DNA
Tőkés-Füzesi M. , Berki T. , Vincze Á. , Tóth K. , Hegyi ,
1,2
7
2,3
synthesis and methylation reactions could be a preventive
P. , Bajor J. M. , Berki T. , Vincze Á. , Tóth K. , Hegyi
Tőkés-Füzesi 5 7 8 2,5 2,3 synthesis and methylation reactions could be a preventive
step against oncogenic processes. Furthermore, tumor origin
1,2
5
1,2
P. , Bajor J. step against oncogenic processes. Furthermore, tumor origin
and heterogeneity could influence the cell response depend
1. Institute for Translational Medicine, Medical School,
on experimental models (human SW480 versus animal-
1. Institute for Translational Medicine, Medical School, and heterogeneity could influence the cell response depend
University of Pécs, Pécs, Hungary; 2. János Szentágothai
derived MCA38 colon adenocarcinoma). 80 versus animal-
University of Pécs, Pécs, Hungary; 2. János Szentágothai on experimental models (human SW4
Research Center, University of Pécs, Pécs, Hungary; 3.
Research Center, University of Pécs, Pécs, Hungary; 3. derived MCA38 colon adenocarcinoma).
Division of Cardiology and Angiology, First Department of
Aims: Our aims were to investigate the effect of elevated
Division of Cardiology and Angiology, First Department of Aims: Our aims were to investigate the effect of elevated
Medicine, Medical School, University of Pécs, Pécs,
dNTP pool and modified DNA fragments on tumor growth,
Medicine, Medical School, University of Pécs, Pécs, dNTP pool and modified DNA fragments on tumor growth,
furthermore to detect the influence of different types of
Hungary; 4. Institute of Bioanalysis, Medical School,
furthermore to detect the
Hungary; 4. Institute of Bioanalysis, Medical School, folates on tumor formation. influence of different types of
University of Pécs, Pécs, Hungary; 5. Division of
University of Pécs, Pécs, Hungary; 5. Division of Methods: C57BL/6 (n=35) mice with implanted MCA38
folates on tumor formation.
Gastroenterology, First Department of Medicine, Medical
Gastroenterology, First Department of Medicine, Medical Methods: C57BL/6 (n=35) mice with implanted MCA38
School, University of Pécs, Pécs, Hungary; 6. Division of
mouse colorectal adenocarcinoma cells were divided into 7
School, University of Pécs, Pécs, Hungary; 6. Division of groups. Mice were injected subcutaneously on every 2nd
Hematology, First Department of Medicine, Medical School,
mouse colorectal adenocarcinoma cells were divided into 7
University of Pécs, Pécs, Hungary; 7. Department of
Hematology, First Department of Medicine, Medical School, days over 4 weeks in the doses of 10ug/animal with the
groups. Mice were injected subcutaneously on every 2nd
Laboratory Medicine, Medical School, University of Pécs, days over 4 weeks in the doses of 10ug/animal with the
following materials: dNTP (Group1) and methylated
University of Pécs, Pécs, Hungary; 7. Department of
Pécs, Hungary; 8. Department of Immunology and
Laboratory Medicine, Medical School, University of Pécs, following materials: dNTP (Group1) and methylated
(d5mCTP) deoxynucleotide solution (Group2). In Group 3-5,
Pécs, Hungary; 8. Department of Immunology and (d5mCTP) deoxynucleotide solution (Group2). In Group 3-5,
mice were injected with artificially methylated and
Biotechnology, Medical School, University of Pécs, Pécs,
Biotechnology, Medical School, University of Pécs, Pécs, mice were injected with artificially methylated and
Hungary
unmethylated spleen-derived DNA in different doses. Group
Hungary unmethylated spleen-derived DNA in different doses. Group
6 was supplemented with 20mg/kg folic acid orally, while
Introduction: Patients with coeliac disease (CeD) are 6 was supplemented with 20mg/kg folic acid orally, while
saline (0.9% NaCl) was used as a vehicle in the control
Introduction: Patients with coeliac disease (CeD) are saline (0.9% NaCl) was used as a vehicle in the control
group. In parallel, human-derived SW480 colon cancer cells
exposed to a higher risk of cardiovascular events in which
exposed to a higher risk of cardiovascular events group. In parallel, human-derived SW480 colon cancer cells
haemorheological alterations may play a key role. in which
6
(5x10 ) were injected subcutaneously on SCID mice (n=15).
haemorheological alterations may play a key role. (5x10 ) were injected subcutaneously on SCID mice (n=15).
6
Aims: Our study aimed to assess the haemorheological
Xenograft mice were divided into 3 groups. 20mg/kg folic
Aims: Our study aimed to assess the haemorheological Xenograft mice were divided into 3 groups. 20mg/kg folic
acid (Group A) and 20mg/kg L-methylfolate (Group B) were
profile of CeD patients with a case-control study.
profile of CeD patients with a case-control study. acid (Group A) and 20mg/kg L-methylfolate (Group B) were
Methods: After obtaining informed consent, we collected
supplemented orally 5 times a week, as well as control mice
Methods: After obtaining informed consent, we collected supplemented orally 5 times a week, as well as control mice
clinical data including medical history, signs and symptoms
were treated with saline. The length and width of the
and cardiovascular risk factors, followed by the implanted tumors were measured with sliding calipers two
were treated with saline. The length and width of the
clinical data including medical history, signs and symptoms
implanted tumors were measured
measurement of routine laboratory and hemorheological
and cardiovascular risk factors, followed by the times weekly by the same observer. with sliding calipers two
parameters. Erythrocyte aggregability and deformability Results: In the animal-derived (MCA38) tumor model,
measurement of routine laboratory and hemorheological
times weekly by the same observer.
parameters. Erythrocyte aggregability and deformability Results: In the animal-derived (MCA38) tumor model,
(measured by the LORCA), whole blood viscosity, and
slower tumor developing tendency was observed in the
(measured by the LORCA), whole blood viscosity, and dNTP supplementation group; d5mCTP supplementation
plasma viscosity (measured by the Brookfield viscometer)
slower tumor developing tendency was observed in the
plasma viscosity (measured by the Brookfield viscometer) dNTP supplementation group; d5mCTP supplementation
moderately decreased the tumor growth. Non-methylated
were determined. Dietary adherence of CeD patients was
were determined. Dietary adherence of CeD patients was moderately decreased the tumor growth. Non-methylated
DNA has also a concentration-dependent growth decreasing
assessed with dietary interview. For univariate analysis, we
assessed with dietary interview. For univariate analysis, we DNA has also a concentration-dependent growth decreasing
effect in MCA38 tumor development. However, folate
performed two-sample t-test or Mann-Whitney test, linear
performed two-sample t-test or Mann-Whitney test, linear effect in MCA38 tumor development. However, folate
regression; for multivariate analysis, we applied the random
supplementation has a different effect on tumor development
regression; for multivariate analysis, we applied the random supplementation has a different effect on tumor development
forest model.
based on the origin of the implanted colon tissue. Slower
forest model. based on the origin of the implanted colon tissue. Slower
tumor developing tendency could be detected in the case of
Results: After matching by age and sex, 50 CeD patients
Results: After matching by age and sex, 50 CeD patients tumor developing tendency could be detected in the case of
C57BL/6-C38 tumor model compared to controls, while folate
and 50 controls were included. Demography was similar in
and 50 controls were included. Demography was similar in C57BL/6-C38 tumor model compared to controls, while folate
supplementation stimulated the growth and proliferation of
both groups. Erythrocyte deformability at higher shear stress
tumor cells in SW480-derived xenograft model (p<0.05). of
both groups. Erythrocyte deformability at higher shear stress supplementation stimulated the growth and proliferation
(3-30 Pa) was significantly impaired in CeD compared to
Conclusion: Our study described the effect of differently
(3-30 Pa) was significantly impaired in CeD compared to tumor cells in SW480-derived xenograft model (p<0.05).
controls. We did not find any difference in parameters related
controls. We did not find any difference in parameters related Conclusion: Our study described the effect of differently
methylated DNA fragments and precursor molecules on the
to aggregability, plasma viscosity (1.24±0.16 mPa·s vs
growth of tumor transplants. According to our results, tumor
to aggregability, plasma viscosity (1.24±0.16 mPa·s vs
1.27±0.15 mPa·s, p=0.209) and whole blood viscosity methylated DNA fragments and precursor molecules on the
1.27±0.15 mPa·s, p=0.209) and whole blood viscosity growth of tumor transplants. According to our results, tumor
(4.04±0.43 mPa·s vs 4.14±0.43 mPa·s, p=0.347).
volume decrease was observed by providing sufficient DNA
Adherence to gluten-free diet significantly correlated with components to normal synthesis. Based on our results folate
(4.04±0.43 mPa·s vs 4.14±0.43 mPa·s, p=0.347).
volume decrease was observed by providing sufficient DNA
aggregability parameters [i.e. aggregation index (r=-0.36,
components to normal synthesis. Based on our results folate
Adherence to gluten-free diet significantly correlated with treatment has a different effect on cancer development
aggregability parameters [i.e. aggregation index (r=-0.36, treatment has a different effect on cancer development
p=0.009), γ (r=-0.59, p<0.001), t1/2 (r=+0.28, p=0.046)],
regarding the tumor origin. Folate has a key role in nucleotide
p=0.009), γ (r=-0.59, p<0.001), t1/2 (r=+0.28, p=0.046)], regarding the tumor origin. Folate has a key role in nucleotide
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Central European Journal of Gastroenterology and Hepatology 71
Volume 6, Supplementum 2 / November 2020